Nencki Institute of Experimental Biology offers following custom research services::

1. Laboratory of Flow Cytometry

- Expertise and technical assistance in flow cytometry,
- cell sorting as well as laser scanning cytometry
- Immunophenotyping
- Analysis of apoptosis and viability
- Analysis of proliferation, cell cycle and mitosis
- Analysis of DNA damage/breaks
- Analysis of calcium ions, free radicals production and mitochondrial membrane potential
- Immunofluorescence of surface and intracellular markers expression
- Cytokine production
- Others, designed individually, dependently on needs.
- Expert consultation for experiment design, fluorochrome selection and data analysis
- Education – we organise lectures, training courses and hands - on workshops for beginners and advanced researchers
Contact: Katarzyna Piwocka, PhD – k.piwocka@nencki.gov.pl

2. Confocal and Fluorescence Microscopy Laboratories

- Complete photographical service is available
- Expertise in the field of image processing and analysis
- Assistance and advice in use of the equipment
IBB PAS contact: Anna Anielska-Mazur, PhD – aam@ibb.waw.pl;
Nencki Institute contact: Prof. Wanda KÅ‚opocka – wklopocka@nencki.gov.pl
IIMCB contact: Tomasz WÄ™gierski, PhD – twegiereski@iimcb.gov.pl

3. Laboratory of Electron Microscopy

- Complete photographical service is available
- Service in the procedure of mild drying of the biological samples in liquid CO2
(critical point), coating of the dried biological samples with neutral metals for scanning microscopy and carbon evaporation of the formvar-coated specimen grids for transmission electron microscopy and microanalysis.
Contact: Prof. Elżbieta Wyroba – e.wyroba@nencki.gov.

4. Laboratory of Cell Engineering

- Genetically Modified Animal Models: preparing constructs (choosing the promoter, cloning, confirming the expression by in vitro assays), microinjection of zygotes, oviduct transfer of the injected zygotes into pregnant/pseudopregnant females, vasectomy in males, tail or ear tissue biopsy, DNA isolation, genotyping
- Viral vectors technology: expertise and technical assistance regarding choosing of the tissue specific promoters, projecting and producing vectors carrying
- silencing sequences, cloning, preparing the plasmids for production of viruses,
- production of the viral vectors (lenti, adenoviral), titer analysis (Real-Time PCR),
- further experiments with created viral vectors (e.g. developing of stable cell lines by use of lentiviral vectors)
Contact: Agata Klejman, PhD, – a.klejman@nencki.gov.

5. Project MIND

- Behavioural phenotyping of animals (mice and rats)
- Expertise on behavioural testing and assistance on behavioural experiments design
- Behavioural analysis
- Drugs' side effects analysis, including subtle changes not detected by traditional means (unique software)
- Evaluation of therapeutic methods
- Drugs addictive effects analysis
- IntelliCage environment for mice behavior analysis
- Evaluation of impact of central nervous system injuries
Contact: PaweÅ‚ Boguszewski, PhD – p.boguszewski@nencki.gov.pl

6. Laboratory of Preclinical Studies in Neurodegenerative Diseases

- Characteristics of potential drugs on animal model of human taupathies (ex. Alzheimer's disease in mice and rats)
- Safety pharmacology (in nervous, vascular and respiratory systems)
- Toxicology (blood diagnostics, clinical biochemistry, histopathology)
- Pharmacokinetics
Contact Person: Grażyna Niewiadomska, PhD – g.niewiadomska@nencki.gov